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June 2008
As a second stably transfected cell line expressing the Ca2+-sensor Case12, PC-12 cells as dopaminergic neuron precursors have been generated expressing cytoplasm-targetted Case12. It has become now possible to use such neurons, differentiated by means of nerve-growth-factor, for the study of Ca2+-mediated neurotransmittter release. PC-12 rat phaeochromocytoma cells expressing green fluorescent Case12-Ca-indicator The main second messenger in the cell, ionized Ca2+, has become visible in the living cell! MARINPHARM has generated the first stably transfected cell line expressing Case12-protein. Case12 is a green fluorescent protein that increases reversibly its fluorescence with increasing Ca2+-concentrations and these fluorescence changes occur within the physiological range of µmolar Ca2+-concentrations. To demonstrate the sensitivity of the sensor, view increased Ca2+-concentration in the living mitotic cell at the poles!
U-20s-5 human osteosarcoma expressing green fluorescent Case12-Ca-indicator Other new cell lines : U-20s-5 human osteosarcoma expressing KillerRed targeted to the plasma membrane HTB-65 human melanoma expressing the far-red fluorescent protein Tag-FP635 U-20s-5 human osteosarcoma expressing the far-red fluorescent protein Tag-FP635 Human melanoma A-375, a widely used model cell system in preclinical research, expressing red fluorescent KillerRed targetted to the plasma membrane Human melanoma HTB-65, another widely used tumor model in the nude mouse, expressing red fluorescent KillerRed targetted to the plasma membrane
Red fluorescent centrosomes in human osteosarcoma U-20s-5, based on a mutation in human alpha tubulin Microtubules are now available either in green , red , or yellow . Recently generated cell lines (ideal tool to follow ROS-, especially H2O2-, changes in real time): U-20s-5 human osteosarcoma expressing green fluorescent Hyper-mitochondria U-20s-5 human osteosarcoma expressing green fluorescent HyPer protein in the cytoplasm
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New far-red fluorescent proteins have been developed allowing excellent opportunities for imaging cells in live animals; ideal tools for many preclinical investigations.
The proteins are described in the following publication:
Shcherbo, D. et al.: Bright far-red fluorescent protein for whole-body imaging. Nature Methods, 4, 741-746 (2007).
MARINPHARM has already generated the following cell lines stably expressing these new red-fluorescent proteins:
Ehrlich-Ascites mouse tumor cells-FP635 Human uterus carcinoma HeLa-FP602 metastasizing human melanoma Mel-Juso-FP602 metastasizing human melanoma Mel-Juso-FP635 Tools for apoptosis research: Human osteosarcoma expressing JRed-BID AND TurboGreen Mitochondria Human osteosarcoma U-20s-5 expressing TurboGreen-Mitochondria Human osteosarcoma U-20s-5 expressing JRed-Mitochondria Human osteosarcoma U-20s-5 expressing yellow fluorescent actin Human osteosarcoma U-20s-5 expressing green fluorescent actin Human osteosarcoma U-20s-5 expressing red fluorescent actin MDCK canine kidney epithelium expressing yellow fluorescent microtubules Human osteosarcoma U-20s-5 expressing blue fluorescent mitochondria |
